5 edition of The biophysical characterisation of the cell surface found in the catalog.
|Statement||G. V. Sherbet.|
|LC Classifications||QH601 .S43|
|The Physical Object|
|Pagination||xviii, 298 p. :|
|Number of Pages||298|
|LC Control Number||77015334|
Schuck P., Zhao H. () The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing. In: Mol N., Fischer M. (eds) Surface Plasmon Resonance. Methods in Molecular Biology (Methods and Protocols), vol Humana Press. First Online 22 January Cited by: Use of an AFM probe functionalized by a peptidic ligand allows mapping of the cognate GPCR on a cell surface, as demonstrated for the calcitonin receptor on live rat osteoclasts using an AFM cantilever attached to a glass sphere functionalized with calcitonin (Lehenkari et al. ). Although AFM studies probing GPCR-ligand interactions are limited at present, these studies are indicative of the potential of Cited by:
The biophysical characteristics of cells determine their shape in isolation and when packed within tissues. Cells can form regular or irregular epithelial structures, round up and form clusters, or deform and attach to substrates. The acquired shape of cells and tissues is a consequence of (i) internal cytoskeletal processes, such as actin polymerisation and cortical myosin contraction, (ii Cited by: •One interacting partner (“ligand”) is attached to the surface of a chip •The second interaction partner (“analyte”) is flown over the surface of the chip. • Binding of molecules to the sensor surface generates a response that is proportional to the bound mass.
Enolase, which catalyses the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, is an important enzyme in the classic glycolysis pathway in cells. Enolase is highly conserved in organisms from bacteria to humans, indicating its importance in cells. Thus, enolase is a good target for developing new drugs. In the last decade, new functions of this enzyme have been found. Helicobacter Cited by: 1. Recent discoveries suggest that flagellar motors are able to remodel in response to a variety of environmental stimuli and are among the triggers for surface colonization and infections. The precise mechanisms by which motors remodel and promote cellular adaptation likely depend on key motor by: 4.
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Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by : J. Chayen. Additional Physical Format: Online version: Sherbet, G.V. (Gajanan V.). Biophysical characterisation of the cell surface.
London ; New York: Academic Press, Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or.
SARS-CoV-2 is a novel highly virulent pathogen which gains entry to human cells by binding with the cell surface receptor angiotensin converting enzyme (ACE2). We computationally contrasted the binding interactions between human ACE2 and coronavirus spike protein receptor binding domain (RBD) of the epidemic-causing SARS-CoV-1, SARS-CoV-2, and bat coronavirus.
In book: Development of Biopharmaceutical Drug-Device Products, pp The goal is to make a comprehensive B-cell epitope map of the surface of ricin, and to understand what makes an. As a result, and because such changes to the biopharmaceutical manufacturing process (e.g., raw material and/or cell line changes to cell culture,) are inevitable, to safeguard against the possibility that a manufacturing process change alters the HOS of a drug, biophysical characterization, especially with high-resolution methods at key Author: Damian J.
Houde, Steven A. Berkowitz. To obtain a comprehensive understanding of the native properties of nanodiscs, modification-free analysis methods are required. In this work we provide a strategy, using a combination of dynamic light scattering and analytical ultracentrifugation, for the biophysical characterization of unmodified by: The biophysical characterization of CPX, a liposomal formulation of cytarabine and daunorubicin encapsulated in a synergistic molar ratio (respectively), is presented.
CPX is a promising drug candidate currently in two concurrent Phase 2 trials for treatment of acute myeloid by: Cells live in a complex and dynamic microenvironment, and a variety of microenvironmental cues can regulate cell behavior. In addition to biochemical signals, biophysical cues can induce not only immediate intracellular responses, but also long-term effects on phenotypic changes such as stem cell differentiation, immune cell activation and somatic cell : Yang Song, Yang Song, Jennifer Soto, Binru Chen, Li Yang, Song Li.
Hence, a robust set of biophysical techniques to characterize and assess the “higher order structure” (HOS) of monoclonal antibodies (mAbs) is an integral part of the drug development process.
From cell biology, evidence favoring the importance of “mutual aid” for evolution generally, and for human social-ethical evolution in particular, was presented in the last two chapters of THE BIOLOGY OF THE CELL SURFACE (Philadelphia: P Blakiston’s Son, a) by 5/5(2).
Journals & Books; Register Sign in. Thus, these biologically orthogonal nanoparticles probe the biophysical properties of the cell at a length scale that was previously challenging to study. GEMs Allow Rapid Characterization of the Rheological Properties of the Cytosol in Yeast and Human by: Title:Biophysical Characterisation and Quantification of Nucleic Acid-Protein Interactions: EMSA, MST and SPR VOLUME: 16 ISSUE: 8 Author(s):Janine K.
Flores, Ruvini Kariawasam, Adrian X. Gimenez, Stephanie Helder, Liza Cubeddu, Roland Gamsjaeger and Sandro F. Ataide Affiliation:School of Molecular Bioscience, University of Sydney, NSW,Australia and School of Science and Health Cited by: 3.
Our in vitro biochemical data showed that idelalisib potently and selectively inhibits the kinase activity of PI3Kδ, and the kinetic characterization of inhibition demonstrated that it is competitive with ATP.
The kinetic analysis, along with the dilution and surface plasmon resonance experiments, clearly showed that idelalisib binds reversibly to the by: Cell surface small molecules and macromolecules, such as members of cholesterol family (including steroid hormones), the glycolipid family (sphingolipids), the glycoprotein family (both N-linked and O-linked), and a vast array of other receptors have been shown to be involved in normal and abnormal cellular processes.
Synthesis and Biophysical Characterization of the Chlorosulfolipids of Ochramonas danica. Grace M. McKenna, control of cell surface interactions, I will review work from my laboratory beginning with mechanistic studies relating to the biophysical basis of cell shape determination and tissue morphogenesis, and ending with demonstration.
Biophysical Characterization. Located on the 5th floor in the Life Science Laboratories the Biophysical Characterization facility houses 15 state-of-the-art instruments for the study of structures and interactions of biological macromolecules such as proteins, nucleic acids, lipids, and complexes.
Understanding the biophysical interactions of nanoparticles (NPs) with cell membranes is critical for developing effective nanocarrier systems for drug delivery applications. We developed an endothelial model cell membrane (EMM) using a mixture of lipids and Langmuir balance to study its interaction with NPs.
Polystyrene NPs of different surface chemistry and sizes were used as a model Cited by: Title:Biophysical Characterisation and Quantification of Nucleic Acid-Protein Interactions: EMSA, MST and SPR VOLUME: 16 ISSUE: 8 Author(s):Janine K. Flores, Ruvini Kariawasam, Adrian X.
Gimenez, Stephanie Helder, Liza Cubeddu, Roland Gamsjaeger and Sandro F. Ataide Affiliation:School of Molecular Bioscience, University of Sydney, NSW,Australia and School of Science and Health. Biophysical Techniques. The characterization of molecular structure, the measurement of molecular properties, and the observation of molecular behavior presents an enormous challenge for biological scientists.
A wide range of biophysical techniques have been developed to study molecules in crystals, in solution, in cells, and in organisms. Abstract. Thermodynamic parameters characterizing the interaction of O-lauroylcholine (OLC) and O-myristoylcholine (OMC) with PDC have been determined by isothermal titration calorimetric ion of OLC or OMC with PDC resulted in exothermic peaks, the magnitude of which decreased with subsequent injections, showing saturation of the binding sites at high protein Cited by: 2.In the last 20 years, an increasing number of studies have been reported on membrane active peptides.
These peptides exert their biological activity by interacting with the cell membrane, either to disrupt it and lead to cell lysis or to translocate through it to deliver cargos into the cell and reach their target. Membrane active peptides are attractive alternatives to currently used Cited by: Biophysical characterization of antibodies with isothermal titration calorimetry.
Cell-surface glycans and polysaccharides have been stud- Biophysical characterization of antibodies : Verna Frasca.